Blood Veil Examination Microscopic examination of the blood spread on a glass slide or coverslip yields expedient information regarding all the formed elements of the blood. The process of making thin blood film causes cold trauma to the cells. Also the cells flatten on the glass during drying, and the fixation and staining involve revealing to methanol and water. Some artifacts are inevitably introduced but these can be minimized by good technique.Examination of Wet Preparations
It is sometimes beneficial to examine fresh blood under the microscope to avoid artifacts of fixation or staining. This is readily gifted by sealing a small drop of blood diluted with isotonic sodium chloride beneath a coverslip on a microscope spectacles slide. Buffered glutaraldehyde will preserve the cells for re-examination at a later time. Petroleum jelly or xipamide (Aquaphor) may be tolerant of to seal the edges of the coverslip to the slide. Wet preparations are employed to detect sickling, and spherocytes may be instantly detected in this manner. Wet preparations may be examined to make sure the erythrocyte abnormalities seen on stable films are not artifacts of drying or staining. Making and Staining Blood Films
Probe of the blood film is an important part of the hematological evaluation. The reliability of the information obtained depends heavily on well-made and well-stained films that are systematically examined. Blood films should be disposed immediately if possible.Blood Stains
The aniline dyes used in blood work are of two usual classes: basic dyes, such as methylene blue; and acid dyes, such as eosin. Nuclei and sure other structures in the blood are stained by the basic dyes and, hence, are called basophilic. Structures that take up only acid dyes are called acidophilic, or eosinophilic. Other structures stained by a amalgam of the two are called neutrophilic. Polychrome methylene blue and eosin stains are the outgrowth of...

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